| Lab Skills |
Computer Skills |
- Animal cell culture
- Karyotyping
- Fluorescence microscopy
- Liquid scintillation/quench curve
- Immunoassay
- Restriction mapping
- Cloning into plasmids & lambda phage & screening/selection of transformed clones
- Ames test
- Bacterial/fungal strain characterization
- Biochemistry of iron
- Collection management
- Phage plaque assay & deletion mapping
- PCR, Sanger sequencing, cycle sequencing
- Southern blot, Northern blot
- Western blot, SDS-PAGE (silver stain)
- Chromatography (HPLC isocratic & gradient columns)
- Gel filtration, affinity column
- Dialysis, salting in/out
- Edman degradation
- Fiske-Subbarow phosphate assay
- Bradford protein assay
- Lowry protein assay
- Enzyme kinetics (linear & non-linear regression) & calculation of enzyme specific activity
- Apparatus fabrication
- Biochem/molecular biology computer apps (ChemDraw, NIH Image, RasMol, Hitachi DNASIS suite, GCG, BLAST, etc.).
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Applications
Word processing & publishing (Word, FrameMaker, PageMaker, Quark Xpress), graphics (PhotoShop, Illustrator), video editing (Ulead), database (Excel, incl. math & stats functions), biochem/molecular (ChemDraw, NIH Image, RasMol, GCG, BLAST, Prizm, Kaleidagraph, etc.)
Platforms
DOS, Win (XP, NT), Mac OS X, UNIX/Linux
Programming
Basic coding skills in Java, C, HTML, XML, Perl; have showable portfolio of C and Java applets and command-line applications (prime number generator, power series generator, color palette, any-sided polygon, etc.)
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